ABSTRACT
Objective@#To evaluate amniotic fluid cell inheritance and re-culture in cases of amniotic fluid with abnormal traits.@*Methods@#From January 2014 to December 2018, 15 cases of amniotic fluid with abnormal traits in the First Affiliated Hospital of Anhui Medical University were selected in amniocentesis.Amniotic fluid cells were routinely seeded and cultured for 10 days, then subcultured into other culture bottles.The number of cells and karyotyping after harvesting were counted.@*Results@#Seven of 15 cases of amniotic fluid color were slightly darker, 3 cases were pink as water washed meat, and 5 cases were light brown or brown.The average number of cells in original bottles was (2.40±5.87)×105/mL, the average number of cells in inheritance bottles was (2.76±0.64)×106/mL.All 15 samples in the cell inheritance bottles got satisfactory results in cell karyotype analysis.@*Conclusion@#Amniotic fluid cell inheritance and re-culture can increase the number of cells in amniotic fluid cell culture and improve the success rate of karyotyping.
ABSTRACT
Objective To evaluate amniotic fluid cell inheritance and re －culture in cases of amniotic fluid with abnormal traits.Methods From January 2014 to December 2018,15 cases of amniotic fluid with abnormal traits in the First Affiliated Hospital of Anhui Medical University were selected in amniocentesis .Amniotic fluid cells were routinely seeded and cultured for 10 days,then subcultured into other culture bottles.The number of cells and karyotyping after harvesting were counted.Results Seven of 15 cases of amniotic fluid color were slightly darker ,3 cases were pink as water washed meat ,and 5 cases were light brown or brown.The average number of cells in original bottles was (2.40 ±5.87)×105／mL, the average number of cells in inheritance bottles was (2.76 ±0.64)×106／mL.All 15 samples in the cell inheritance bottles got satisfactory results in cell karyotype analysis .Conclusion Amniotic fluid cell inheritance and re－culture can increase the number of cells in amniotic fluid cell culture and improve the success rate of karyotyping.
ABSTRACT
Objective To probe into the pathogenesis of gestational diabetes mellitus (GDM) by studying expression of TLR4 acetylation in peripheral blood mononuclear cells of gravida with GDM as well as its role in TLR4 inflammatory pathway. Methods 30 normal gravidas and 30 gravidas with GDM were enrolled in the study, 15 mL peripheral blood from every participant was collected for extraction of mononuclear cells via density gradient centrifugation and culturing in vitro by LPS. The expression of TLR4 acetylation in the mononuclear cells was detected using immunoprecipitation and western blot and the levels of inflammatory cytokines, TNF-α, IL-1 and IL-10 in the supernatant were detected by ELISA. Results TLR4 acetylation appeared positive in GDM group, and negative in the control group. After the LPS intervention, the degree of TLR4 acetylation was enhanced significantly and the latter was significantly higher than those of the GDM group and the normal + Lps group (P < 0.05). The differences in the levels of inflammatory cytokines, TNF-α, IL-1, IL-10, were statistically significant (P < 0.05). Conclusion TLR4 acetylation exists in mononuclear cells of gravida with GDM, and mediates the release of inflammatory cytokines by influencing the activation of TLR4 inflammatory pathobenesis, which contributes to the promoted antiinflammatory - proinflammatory imbalance in gravidas. In this way, it is involved in the growth of GDM.